SBEMimage is an open-source Python application to run acquisitions with scanning electron microscopes, in particular for serial block-face imaging. Read the paper.

The latest version is 2020.12. For users who are not familiar with Python, it is recommended to download the Windows 7/10 installer.

The development of SBEMimage at the Friedrich Miescher Institute in Basel has been supported by the Novartis Research Foundation and by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant Agreement No. 742576). Other institutes that have substantially contributed to SBEMimage development/testing: EPFL, Lausanne, Switzerland (CIME/BioEM); Francis Crick Institute, London, UK.

Current development team: Benjamin Titze (btitze), Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland (lead developer); Thomas Templier, Janelia Research Campus; Joost de Folter, Francis Crick Institute; Philipp Schubert, Max Planck Institute of Neurobiology; and others.

Contact btitze ÄT protonmail.ch if you are interested in contributing to the development of SBEMimage. All ongoing development takes place in the ‘dev’ branch. Pull requests to that branch are welcome. For more information, see the section ‘For developers’ in this user guide.

Please use GitHub Issues for bug reports. For general feedback or feature suggestions, post to the Image.sc forum with the tag ‘sbemimage’, or send an email to btitze ÄT protonmail.ch.

Please cite the following paper if you use SBEMimage:

Titze B, Genoud C and Friedrich RW (2018) SBEMimage: Versatile Acquisition Control Software for Serial Block-Face Electron Microscopy. Front. Neural Circuits 12:54. doi: 10.3389/fncir.2018.00054

SBEMimage is licensed under the MIT License.